abstract：:The high concentration of gamma-aminobutyric acid (GABA) recently demonstrated in rat ovary prompted us to examine the capacity of ovarian slices to take up [3H]GABA. Active uptake, dependent on temperature and sodium concentration, was observed and a kinetic constant (Km) of 1.0 microM found for the uptake process. O...

journal_title：Journal of neurochemistry

authors： Erdö SL

更新日期：1983-02-01 00:00:00

abstract：:Lipid precursors ([2-3H]glycerol for phospholipids and [3H]acetate for cholesterol) were injected into the L-5 dorsal root ganglion of adult rats. At various times, animals were killed, the ganglion and consecutive 5-mm segments of sciatic nerve were dissected, and lipids were extracted and analyzed by TLC. Individual...

journal_title：Journal of neurochemistry

authors： Toews AD,Saunders BF,Blaker WD,Morell P

更新日期：1983-02-01 00:00:00

abstract：:Ammonia, amino acids (AA), and middle molecules (MM) have been implicated in the pathogenesis of experimentally induced acute hepatic coma in the pig. Hemodialysis (HD) using either a low- (Cuprophan = CU) or a high-permeability (polyacrylonitrile = AN 69) membrane has demonstrated the role of MM. Selective hemodialys...

journal_title：Journal of neurochemistry

authors： Denis J,Delorme ML,Boschat M,Nordlinger B,Opolon P

更新日期：1983-01-01 00:00:00

abstract：:The cellular localization of the two forms of catechol-O-methyltransferase (COMT) was investigated by measuring their activities in rat striatum following unilateral stereotaxic injection of kainic acid, which causes degeneration of striatal neurons followed by proliferation of astroglial cells. Membrane-bound COMT ac...

journal_title：Journal of neurochemistry

authors： Rivett AJ,Francis A,Roth JA

更新日期：1983-01-01 00:00:00

abstract：:We report the distribution of choline acetyltransferase (ChAT) activity in the laminae of the rat olfactory tubercle. Within its posterior medical portion, the tubercle contains three parallel histological laminae that can be separated by cutting tangential sections from frozen tissue. ChAT was measured in homogenates...

journal_title：Journal of neurochemistry

authors： Gordon CR,Krieger NR

更新日期：1983-01-01 00:00:00

abstract：:Oxidation of ketone bodies (KBs) generates acetyl coenzyme A (AcCoA), which can be further incorporated into fatty acid. We have determined the rates of lipogenesis from ketone bodies in developing rats and their relation to the activities of enzymes involved in the production of cytoplasmic AcCoA via different pathwa...

journal_title：Journal of neurochemistry

authors： Yeh YY,Ginsburg JR,Tso TB

更新日期：1983-01-01 00:00:00

abstract：:The intraaxonal transport of phospholipids in regenerating hypoglossal nerve of the rabbit was investigated by administration of labeled lipid precursors into the medulla oblongata. At various time intervals after crushing the left hypoglossal nerve at the level of the digastric muscle, a mixture of 60 mu Ci of [2-3H]...

journal_title：Journal of neurochemistry

authors： Alberghina M,Viola M,Giuffrida AM

更新日期：1983-01-01 00:00:00

abstract：:A Ca2+- and calmodulin-dependent protein kinase was purified from rat brain cytosol fraction to apparent homogeneity at approximately 800-fold and with a 5% yield. The purified enzyme had a molecular weight of 640,000 as determined by gel filtration analysis on Sephacryl S-300 and a sedimentation coefficient of 15.3 S...

journal_title：Journal of neurochemistry

authors： Fukunaga K,Yamamoto H,Matsui K,Higashi K,Miyamoto E

更新日期：1982-12-01 00:00:00

abstract：:The carbohydrate content of human brain glycoproteins was studied during development from the age of 12 fetal weeks to 8 postnatal months. The concentration of all the glycoprotein monosaccharides increased with age. The total amount of glycoprotein monosaccharides per lipid-free dry tissue increased by about 150% fro...

journal_title：Journal of neurochemistry

authors： Korpeinen T,Mononen I,Krusius T,Järnefelt J

更新日期：1982-12-01 00:00:00

abstract：:Myelin was isolated from the brains of 16-day-old rabbits that had received intracerebral injections of [32P]orthophosphate 24 h earlier. The basic protein was extracted and examined by polyacrylamide gel electrophoresis both at low and at high pH. At low pH a single band corresponding to the basic protein contained a...

journal_title：Journal of neurochemistry

authors： Agrawal HC,Martenson RE,Agrawal D

更新日期：1982-12-01 00:00:00

abstract：:Microelectrophoretic methods were used to study the effects on cat spinal neurones of a number of compounds structurally related to the gamma-aminobutyric acid (GABA) agonists muscimol, THIP, and isoguvacine. While N-methylmuscimol was an agonist at bicuculline methochloride-sensitive GABA receptors, somewhat weaker t...

journal_title：Journal of neurochemistry

authors： Krogsgaard-Larsen P,Hjeds H,Curtis DR,Leah JD,Peet MJ

更新日期：1982-11-01 00:00:00

abstract：:An acid-hydrolyzable conjugate of 3,4-dihydroxyphenylethylamine (dopamine, DA) was detected in superfusates from slices from rat striatum. The concentrations of endogenous free and conjugated DA, and of the acid metabolites (3,4-dihydroxyphenylacetic acid [DOPAC] and homovanillic acid [HVA]) in superfusates were measu...

journal_title：Journal of neurochemistry

authors： Tyce GM,Rorie DK

更新日期：1982-11-01 00:00:00

abstract：:The effect of L-phenylalanine on the transport of tyrosine was studied using membrane vesicles from rat brain synaptosomes. Phenylalanine, which is accumulated in phenylketonuria, competitively inhibits tyrosine uptake at concentrations similar to those found in phenylketonuric patients, with a Ki of the same order of...

journal_title：Journal of neurochemistry

authors： Aragón MC,Giménez C,Valdivieso F

更新日期：1982-10-01 00:00:00

abstract：:The anticonvulsant drugs phenobarbital, phenytoin, sodium valproate, and phenytoin-sodium valproate in combination were administered daily to (a) pregnant rats starting on the 5th day after conception, and continued through 17 days postpartum, or (b) to developing rats between 3 and 17 days of age. Each drug was prepa...

journal_title：Journal of neurochemistry

authors： Patsalos PN,Wiggins RC

更新日期：1982-10-01 00:00:00

abstract：:Different forms of acetylcholinesterase (AChE), EC 3.1.1.7, were demonstrated in human brain caudate nucleus. One form was solubilized at high ionic strength, the other with Triton X-100. The detergent-extractable form was purified to homogeneity by affinity chromatography. This form of AChE is amphiphile-dependent; i...

journal_title：Journal of neurochemistry

authors： Sørensen K,Gentinetta R,Brodbeck U

更新日期：1982-10-01 00:00:00

abstract：:Human brain aldose reductase and hexonate dehydrogenase are inhibited by alrestatin (AY 22,284) and sorbinil (CP 45,634). Inhibition by alrestatin is noncompetitive for both enzymes, and slightly stronger for hexonate dehydrogenase (KI values 52-250 microM) than for aldose reductase (KI values 170-320 microM). Sorbini...

journal_title：Journal of neurochemistry

authors： O'Brien MM,Schofield PJ,Edwards MR

更新日期：1982-09-01 00:00:00

abstract：:The dose-dependent effects of two putative gamma-aminobutyric acid (GABA)-ergic agonists, muscimol and 4,5,6,7-tetrahydroisoxazolo(4,5-e)-pyridin-3-ol (THIP), upon local cerebral glucose utilisation in 60 discrete regions of the CNS have been examined in conscious rats using the [14C]2-deoxyglucose quantitative autora...

journal_title：Journal of neurochemistry

authors： Kelly PA,McCulloch J

更新日期：1982-09-01 00:00:00

abstract：:The presence of serine transhydroxymethylase (STHM; EC 2.1.2.1) in crude homogenates of rat, guinea pig and goat retina has been demonstrated. There was a variation in the levels of retinal STHM activity in the three species. Substantial STHM activity was found in all the three retinal preparations in the absence of p...

journal_title：Journal of neurochemistry

authors： Dasgupta P,Narayanaswami A

更新日期：1982-09-01 00:00:00

abstract：:The parenteral administration of L-phenylalanine or p-tyrosine increases the mouse striatal concentration of p-tyramine, an effect that is enhanced by monoamine oxidase inhibition and reduced by an L-aromatic aminoacid decarboxylase inhibitor. Striatal m-tyramine was increased following administration of L-phenylalani...

journal_title：Journal of neurochemistry

authors： Juorio AV,Boulton AA

更新日期：1982-09-01 00:00:00

abstract：:A protein that binds to membranes in a calcium-dependent manner between calcium concentrations of 10(-5) and 10(-6) M has been isolated in large amounts (20 mg/kg tissue) from the entirely cholinergic electric organ of Torpedo marmorata. The protein bound reversibly to membrane fractions in a calcium-specific and satu...

journal_title：Journal of neurochemistry

authors： Walker JH

更新日期：1982-09-01 00:00:00

abstract：:The activities of the three major catecholamine-synthesizing enzymes were determined in brain tissue pellets dissected from 500-micrometers thick horizontal sections of rat lower brainstem. The rostrocaudal distributions of the three enzymatic activities were generally not parallel, suggesting differences in the respe...

journal_title：Journal of neurochemistry

authors： Chamba G,Denoroy L,Renaud B

更新日期：1982-08-01 00:00:00

abstract：:45Ca2+ uptake measurements were performed on intact and osmotically lysed synaptosomes from rat brain to study the possible influence of prostaglandins (PGs) on Ca2+ movements into and within the nerve endings. The K+-induced 45Ca2+ uptake of intact synaptosomes was not influenced by several inhibitors of PG synthesis...

journal_title：Journal of neurochemistry

authors： Denzlinger C,Hertting G,Jackisch R

更新日期：1982-08-01 00:00:00

abstract：:Rats with unilateral lesions of the locus coeruleus were used to study the role of norepinephrine (NE) signal input in the down-regulation by antidepressants of the noradrenergic cyclic AMP-generating system in the cortex. Chronic administration of both desipramine (blockade of NE reuptake) and iprindole (no blockade ...

journal_title：Journal of neurochemistry

authors： Janowsky A,Steranka LR,Gillespie DD,Sulser F

更新日期：1982-07-01 00:00:00

abstract：:Galactosyltransferase activities were examined in the cerebellum, cerebral cortex, and brain stem of reeler and wild-type mice. Galactosyltransferase assays were optimal for all required substrates, linear with incubation time, and proportional to protein concentration. In brain areas affected by the reeler mutation (...

journal_title：Journal of neurochemistry

authors： Shur BD

更新日期：1982-07-01 00:00:00

abstract：:Highly purified human serum cholinesterase (EC 3.1.1.8, also known as pseudocholinesterase and butyrylcholinesterase) had peptidase activity toward substance P. Digestion of substance P was monitored by high performance liquid chromatography, which separated three product peptides. The cleavages occurred sequentially....

journal_title：Journal of neurochemistry

authors： Lockridge O

更新日期：1982-07-01 00:00:00

abstract：:The binding of the potent adenosine uptake inhibitor [3H]nitrobenzylthioinosine ([3H]NBI) to brain membrane fractions was investigated. Reversible, saturable, specific, high-affinity binding was demonstrated in both rat and human brain. The KD in both was 0.15 nM with Bmax values of 140-200 fmol/mg protein. Linear Sca...

journal_title：Journal of neurochemistry

authors： Marangos PJ,Patel J,Clark-Rosenberg R,Martino AM

更新日期：1982-07-01 00:00:00

abstract：:Explants of adrenal medullae were cultured in defined media for up to 48 h, during which time the tissue remained histologically intact. Addition of N6-methyladenosine to the medium led to a diminution in the activity of phenylethanolamine N-methyltransferase (EC 2.1.1.28) in the tissue. The enzyme activity was invers...

journal_title：Journal of neurochemistry

authors： Burke WJ,Joh TH

更新日期：1982-07-01 00:00:00

abstract：:The specific interaction of S-100 protein with synaptosomal particulate fractions (SYN) was further investigated with special reference to the number of binding components and their localization in synaptosomal subfractions. Binding studies were conducted on SYN from various CNS regions, on synaptosomal subfractions f...

journal_title：Journal of neurochemistry

authors： Donato R

更新日期：1982-07-01 00:00:00

abstract：:The hippocampal formation has been extensively research in terms of its putative neurotransmitters, anatomical connections, and behavioral relevance. An aspect of importance is the assessment of apparent neurotransmitter receptors by using receptor binding assays. In the present study, such assays were done in vitro t...

journal_title：Journal of neurochemistry

authors： Dooley DJ,Bittiger H

更新日期：1982-06-01 00:00:00

abstract：:When synaptosomes from rat brain were incubated with S-adenosylmethionine (10(-3) M), noradrenaline uptake and KCl-stimulated release were decreased. These effects were dependent on MgCl2, temperature, and incubation time. We have investigated the enzymatic methylation of phosphatidylethanolamine to give phosphatidylc...

journal_title：Journal of neurochemistry

authors： Fonlupt P,Rey C,Pacheco H

更新日期：1982-06-01 00:00:00

abstract：:Exogenously administered estrogens can enhance the rate of increase of glucose 6-phosphate dehydrogenase activity in rat extensor digitorum longus muscles following denervation. In this communication, we report that the effect of denervation on glucose 6-phosphate dehydrogenase activity is modified by variations in en...

journal_title：Journal of neurochemistry

authors： Schaerf FW,Patz T,Max SR

更新日期：1982-06-01 00:00:00

abstract：:The Na+-dependent synaptosomal uptakes of proline, aspartic acid, glutamic acid, and gamma-aminobutyric acid were strong inhibited by monounsaturated fatty acids. With oleic acid, half-maximal inhibition was observed at about 15 microM. The Na+-independent uptakes of leucine, phenylalanine, histidine, and valine were ...

journal_title：Journal of neurochemistry

authors： Rhoads DE,Kaplan MA,Peterson NA,Raghupathy E

更新日期：1982-05-01 00:00:00

abstract：:The activities of monoamine oxidase A (MAO A) and monoamine oxidase B (MAO B) represent two independent types of substrate binding site, as indicated by experiments with selective inhibitors and also by substrate competition. We have tried to determine whether A and B active sites of human brain and liver MAO are loca...

journal_title：Journal of neurochemistry

authors： White HL,Stine DK

更新日期：1982-05-01 00:00:00

abstract：:The relative importance of acetylcholine, dopamine, endogenous opiates, gamma-aminobutyric acid (GABA), glutamate, glycine, noradrenaline, and serotonin as transmitters in the pigeon visual system was estimated by measuring the activity of choline acetyltransferase (ChAT), glutamic acid decarboxylase (GAD), and aromat...

journal_title：Journal of neurochemistry

authors： Vischer A,Cuénod M,Henke H

更新日期：1982-05-01 00:00:00

abstract：:A protein has been purified from human brain that appears to be the human equivalent of bovine 14-3-3 protein. On polyacrylamide gel electrophoresis the protein migrates as a faster major component, termed 14-3-3-2 protein, and a slower minor component, termed 14-3-3-1 protein, which consists of approximately 12% of t...

journal_title：Journal of neurochemistry

authors： Boston PF,Jackson P,Kynoch PA,Thompson RJ

更新日期：1982-05-01 00:00:00

abstract：:Rat brain succinyl-CoA:3-oxo-acid CoA-transferase (3-oxo-acid CoA-transferase, EC 2.8.3.5), the first committed enzyme in the oxidation of ketone bodies in mitochondria, was purified to apparent homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme has an apparent molecular weight of 90,000 as determ...

journal_title：Journal of neurochemistry

authors： Russell JJ,Patel MS

更新日期：1982-05-01 00:00:00

abstract：:The stimulatory effect of cysteine sulfinic acid on cyclic AMP formation was examined in slices from three different regions of guinea pig brain. The inhibitory effect of taurine on the stimulated formation of cyclic AMP was also studied. Cysteine sulfinic acid (1--10 mM) greatly increased the cyclic AMP level in stri...

journal_title：Journal of neurochemistry

authors： Baba A,Lee E,Tatsuno T,Iwata H

更新日期：1982-05-01 00:00:00

abstract：:Incorporation of [1-14C]palmitic acid into neutral lipids and phospholipids of rat cerebral cortex was examined in vitro in normal Krebs--Ringer bicarbonate buffer containing 3% (wt/vol) albumin and 0.75 mM palmitic acid. Under standard assay conditions, radioactivity in the triacylglycerol fraction increased rapidly ...

journal_title：Journal of neurochemistry

authors： Mizobuchi M,Morisaki N,Matsuoka N,Saito Y,Kumagai A

更新日期：1982-05-01 00:00:00

abstract：:delta 1-Pyrroline, 5-methyl-delta 1-pyrroline, and 5,5-dimethyl-delta 1-pyrroline have been identified as substances metabolized to gamma-aminobutyric acid (GABA), 4-aminopentanoic acid (methylGABA), and 4-amino-4-methylpentanoic acid (dimethylGABA), respectively. An enzyme system residing in the soluble fraction of r...

journal_title：Journal of neurochemistry

authors： Callery PS,Geelhaar LA,Nayar MS,Stogniew M,Rao KG

更新日期：1982-04-01 00:00:00

abstract：:High-affinity binding sites (apparent KD = 1.5 nM) for [3H]desipramine have been demonstrated and characterized in membranes prepared from rat brain. The binding of [3H]desipramine was found to be saturable, reversible, heat-sensitive, sodium-dependent, and regionally distributed among various regions of the brain. Hi...

journal_title：Journal of neurochemistry

authors： Rehavi M,Skolnick P,Brownstein MJ,Paul SM

更新日期：1982-04-01 00:00:00